Sunday, 24 August 2014

Read It and Weep: Fungal Guttation

Guttation on Fomitopsis pinicola bracket fungus
Young Fomitopsis pinicola with guttation drops (click to see bigger)
Some fungi are prone to exhibiting a curious phenomenon—they exude beads of moisture, called guttation. In several polypores, such as Fomitopsis pinicola, the liquid produced can look so much like tears that you'd swear the fungus was weeping. Or maybe sweating. Other species produce pigmented drops that can look like milk, or tar, or even blood.

Guttation is more well-known in some vascular plants. During the night, when the plant's transpiration system is shut down, pressure from excess moisture in the roots can force beads of sap out of special structures on leaf edges. 

strawberry leaf guttation noah erhardt
Guttation droplets on strawberry leaves (Noah Erhardt/Wikipedia)
In fungi, the guttation mechanism is not so well understood. In many species, however, it's so often observed, particularly during times of rapid growth when temperature and humidity are favourable, that these beads of liquid can be a reliable macroscopic characteristic. Hydnellum peckii, for instance, so frequently "bleeds" pigmented drops in its early stages of growth that it's been given gruesome nicknames, including "Bleeding Tooth Fungus" and "Devil's Tooth." Coincidentally, a 1965 study found a compound in the fruiting body of  H. peckii that has anticoagulant properties similar to those of heparin, too much of which can make one bleed to death internally.   

bleeding mushroom guttation lisa neighbour
Producing blood-like guttation droplets during periods of rapid growth is 
well-known characteristic of Hydnellum peckii(Lisa Neighbour)
polypore shelf fungus baby weeping guttation droplets
Young fruit bodies of Fomitopsis pinicola commonly produce copious guttation.
A few weeks ago, I came across a crop of Inonotus glomeratus on a maple log. I've found this amazing polypore a few times, once right after it had showered itself, and everything else around it, with what I assumed were millions of sulphur-yellow spores. The one I found this summer, though, was very young, and instead of spewing spores, it was weeping globules of "tar" in copious enough amounts that shiny black pools were accumulating on the forest floor. Unlike most guttation drops, which are watery, these exudations were thick and sticky and stained my finger and thumb a deep auburn brown. And kind of glued them together. Oddly, though this unusual guttation has been noted by others, there seems to be no mention of it in the literature. I. glomeratus is so unusual in so many ways, I've written a whole post about it.

Inonotus glomeratus fungus dripping black tar guttation
Fast-growing Inonotus glomeratus produces tarry guttation.
yellow spores of polypore Inonotus glomeratus
This Inonotus glomeratus continued to drip its viscous black exudate
even after it began releasing its yellow spores.
holes made guttation Inonotus glomeratus
The guttation drops on this Inonotus glomeratus were so thick that the fungus grew
around them, producing a pitted appearance after rain washed them away.
Polypores and Hydnoids are not the only fungi to produce guttation. In moist conditions, young Suillus americanus stipes can be heavy with yellow-tinted drops. Guttation is also common enough in the uncommon Rhodotus palmatus that this characteristic is often included in descriptions. 


Suillus americanus liquid drops stem
Suillus americanus (above) and Rhodotus palmatus (below) 
guttation of young Rhodotus palmatus

Guttation can happen in incredibly small ways, too. During the Toronto Bioblitz this past spring, we found some Lachnum subvirgineum that, despite what seemed like dry conditions, were covered in minute guttation droplets, as were most other Lachnum I've since come across. 



Lachnum subvirgineum with guttation water droplets
The largest of these Lachnum subvirgineum was less than .5 mm. in 
diameter, which makes the guttation droplets impressively small.
Just yesterday I found another ascomycete sporting the same clear drops—this time Incrucipulum ciliare, whose hair-rimmed discs are even smaller than L. subvirgineum, hence, so, too, were its guttation droplets.


ascomycete oak Incrucipulum ciliare
The guttation droplets on these Incrucipulum ciliare were almost too small for 
my camera to capture. The "stick" in the lower right corner is an oak leaf vein. 
Another minute character is so characteristically bejewelled in guttation droplets, it's named for it: Pilobolus crystallinus, which is one of the "Cannon" or "Hat Thrower" fungi found on herbivore dung.


Pilobilus crystallinus cannon fungus
Pilobilus crystallinus is named for its sparkling
guttation droplets. (
Keisotya/Wikipedia)
Though little is known about guttation in wild fruiting bodies of fungi, it's a common phenomenon of fungal mycelia and hyphae in the lab, and a number of studies have been done to determine what the exudates contain. Penicillin has been found in the guttation droplets produced by Penicillium species in similar concentrations to that found in the culture broth, while gliotoxin, which has immunosuppressive qualities, has been found in guttation droplets of Aspergillus fumigatusDo these fungi use guttation droplets as reservoirs for metabolic byproducts, or do they simply use them for water storage

Or have different species evolved to produce guttation droplets for different purposes? The edible bolete, Suillus bovinus, for instance, has been shown in the lab to reabsorb nutrients from its guttation droplets, while leaving behind less useful byproducts, such as oxalic acid. So perhaps guttation has evolved as an efficient method of expelling waste for some fungi. 

Is that what's going on with Inonotus glomeratus? Is that viscous, black ooze just a collection of rejected metabolic byproducts? If anyone would like to analyze it and has the means, I have some dehydrated exudate that I'd love to send you!  


slime mold Stemonitis flavogenita guttation drops
Even some slime moulds, like this immature Stemonitis 
flavogenita, produce guttation droplets. (Ulrike Kullik) 
pink polypore Fomitopsis rose
Pink-pored Fomitopsis rosea are even prettier when 
decorated with shimmering beads of moisture. I think the 
pattern on rim was made by the teeth of a grazing slug.

young Punctularia strigosozonata bleeding
Punctularia strigosozonata "bleeds" rust-tinted droplets.
Early nubbins of an unidentified polypore exude milky drops.
teardrop shaped indentations left by guttation on bracket fungus
This Fomitopsis pinicola produced guttation droplets for three
months this summer. When it finally stopped, trompe l'oeil
teardrop-shaped indentations were left behind.
Wet weather makes Xylaria hypoxylon produce beads of moisture.
Weeping Pleurotus dryinus
This large Pleurotus dryinus was weeping copiously
despite there having been no rain  for a week.
Many parasitic Hypomyces, such as this H. chrysospermus, are prolific weepers. 
Inonotus dryadeus is a lumpy polypore known for its ample
 production of amber guttation droplets. (Wikipedia)


Selected References:


Erast Parmasto, Andrus Voitk, (2010). Why Do Mushrooms Weep? Fungi, Vol. 3:4
N.B. In my original post, relying on the above article, I had repeated that Fomitopsis pinicola does not produce guttation drops in Newfoundland. Andrus Voitk has since kindly informed me that, in fact, they regularly do, as he notes in Omphalina Vol. III, No. 3. 

Hutwimmer, S., Wang, H., Strasser, H., Burgstaller, W. (2010) Formation of exudate droplets by Metarhizium anisopliae and the presence of destruxins. Mycologia, Vol. 102 no. 1, 1-10

Gerhard Saueracker. On the Exudates of Polypore Fungi. Fungimap Newsletter 48, Jan. 2013

4 comments:

  1. Thanks for the information and the excellent photography. Mark Bower

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  2. Very interesting! I have seen guttation drops on a polypore growing from an aspen. Not sure of the species but do not think it is Phellinus tremulae.

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